Supporting information Nanoscale dynamics of phospholipids reveals optimal assembly mechanism of pore-forming proteins in bilayer membranes

Surface pressure-area isotherm and layer-by-layer phospholipid monolayer transfer for fabrication of SLBs: Surface pressure versus area-per-molecule isotherms were recorded using the KSV LB rectangular mini Trough (area, 240 cm 2 ) equipped with a Wilhelmy balance. A platinum sensor of accuracy 0.1 mN/m was used to measure the interfacial surface pressure. A chloroform solution of either one component DOPC, POPC and DMPC lipid or binary mixture containing varied cholesterol content such as 3:1, 2:1 and 1:1 were spread on the air–water interface of a LB trough at 20 oC using a precise Hamilton syringe to make a compact monolayer. After evaporation of chloroform, the isotherms were recorded at constant temperature and a barrier speed of 5 mm/min.